ANCHOR

Deciphering and controlling DNA processes

The goal of the ANCHOR team is to develop new applications for a live cell DNA biosensor technique based on our recently patented system to investigate several DNA processes. The ANCHOR system is a bipartite system composed of ANCH sequences and OR proteins. ANCH DNA sequences are specifically and independently recognized by OR proteins that nucleate and spread from the ANCH sequence over surrounding DNA. As the OR proteins can be fused to a fluorescent reporter, increased concentration of OR on the ANCH DNA creates a fluorescent focus easily detectable by fluorescence microscopy. We are applying this technology to uncover DNA-based molecular mechanisms in single cells and in high content screening by microscopy on living cells to discover active molecules with broad applications, from bio-production to antiviral activity.


 

ANCHOR technology is of great interest to the research community working on DNA localization and to pharmaceutical industries at large to test effects of new compounds on cancers and virus infection.

Team leader

Kerstin Bystricky

Partner Lab

Key points

Selected references
  • T. Germier, S. Kocanova, N. Walther, A. Bancaud, H. Ahmed Shaban, H. Sellou, A. Z. Politi, J. Ellenberg, F. Gallardo, K. Bystricky – Real-Time Imaging of a Single Gene Reveals Transcription-Initiated Local Confinement. Biophysical Jnal  2017, DOI 10.1016/j.bpj.2017.08.014
  • Lassadi I., Kamgoué A., Goiffon I., Tanguy-le-Gac N. and Bystricky K.. “Differential chromosome conformations as hallmarks of cellular identity revealed by mathematical polymer modeling. ” PLoS Computational Biology, DOI 10.1371/journal.pcbi.1004306, 2015
  • Gallardo F., Saad H., Dalvai M., Lane D. and Bystricky K. “Early steps of double strand break processing revealed by non-intrusive imaging of DNA loci in living cells.” PLoS Genetics 2014. doi:10.1371/journal.pgen.1004187
  • Hajjoul H., Mathon J., Ranchon H., Goiffon I., Mocciconazzi J., Albert B., Carrivain P., Victor J.M., Gadal O., Bystricky K., Bancaud A. “Chromosome dynamics in living yeast: quantitative determination of the physical parameters that govern chromatin movements”. Genome Research, 2013 doi: 10.1101/gr.157008.113
  • Bystricky K., Gallardo F, Lane D, Dubarry N. « Visualisation de la position et de la dynamique de loci d’ADN dans les cellules vivantes en utilisant un nouveau groupe de petits opérateurs bactériens » PCT/EP2012/055258. WO/2012/127047 ; Patent
  • Kocanova S., Kerr E., Rafique E., Boyle S., Caze-Subra S., Bickmore W. and Bystricky K. “Activation of estrogen responsive genes does not require nuclear co-localisation” PloS Genetics, 6(4): e1000922. doi:10.1371, 2010.
Team members
  • Kerstin Bystricky (PU),
  • Bernard Mariame (CR CNRS),
  • Silvia Kocanova (MCU UPS),
  • Thomas Germier (PhD)
Collaborations
  • Startup NeoVirTech, Franck Gallardo
  • Team IP3D. Bernard Ducommun, ITAV
  • Team SoMO, Stefan Chassaing, ITAV
  • 20 public research laboratories worldwide use the ANCHOR technology with MTA to investiguate different aspects of DNA metabolism in living cells.
Patent

Bystricky K., Gallardo F, Lane D, Dubarry N. « Visualisation de la position et de la dynamique de loci d’ADN dans les cellules vivantes en utilisant un nouveau groupe de petits opérateurs bactériens » PCT/EP2012/055258. WO/2012/127047

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