UbiCARE

Understanding Ubiquitin Regulated processes implicated in Cancer

Our team studies processes regulated by members of the ubiquitin family (UbLs) implicated in cancer development. In particular we are interested in understanding the role of these post-translational (PTM) modifications in the response to actual chemotherapy treatments, identify the most important events and molecules implicated in the acquisition of resistance to chemotherapy and evaluate the potential of some of them as biomarkers and/or as drug targets.

 

 

In particular, we explore the remodeling of ubiquitin chain architecture and the role of certain ubiquitin enzymes under these conditions. We are currently studying resistance to Bortezomib (Bzb) in Mantle Cell Lymphoma (MCL) and Acute Myeloid Leukemia (AML) models.

To address many of these questions our group develops molecular traps to isolate, identify, quantify and analyze individual proteins or global cellular events regulated by these PTMs. Our approaches are derived from the necessity to have efficient tools to explore the role of these UbLs on the control of endogenous proteins, not only from cell cultures, but also from animal models and samples from patients.

Team leader

Manuel Rodriguez

Partner Lab

IPBS

Key points

Selected publications
  • Valérie Lang, Fabienne Aillet, Wendy Xolalpa, Sonia Serna, Laurie Ceccato, Rosa G. Lopez-Reyes, Maria Paz Lopez-Mato, Radosław Januchowski, Niels-Christian Reichardt, Manuel S. Rodriguez. (2017) Analysis of defective protein ubiquitylation associated to adriamycin resistant cells. Cell cycle sous presse
  • Serna S., Xolalpa W., Lang V., Aillet F., England P., Reichardt N., and Rodriguez MS. Efficient Monitoring of Protein Ubiquitylation Levels using TUBEs-based Microarrays. FEBS letters 2016 Aug;590(16):2748-56.
  • Mata-Cantero L., Azkargorta M, Aillet F., Xolalpa W., LaFuente MJ, Elortza F., Carvalho AS., Martin-Plaza J., Matthiesen R, and Rodriguez MS. New insights into host-parasite ubiquitin proteome dynamics in P. falciparum infected red blood cells using a TUBEs-MS approach . J Proteomics Apr 29, 2016;139:45-59.
  • Rodriguez MS (Ed), et al SUMO Methods and Protocols. Methods Mol Biol. 1475, Springer 2016
  • Mata-Cantero L, Cid C, Gomez-Lorenzo MG, Xolalpa W, Aillet F, Martín JJ, Rodriguez MS. Development of two novel high-throughput assays to quantify ubiquitylated proteins in cell lysates: application to screening of new anti-malarials. Malar J. 2015 May 14;14:200.
Team members
  • Manuel S. RODRIGUEZ (CR1-CNRS)
  • Laurie BONNAFE (IE CDD-CNRS)
  • Rosa LOPEZ-REYES (PhD CONACyT)
  • Grégoire QUINET (PhD ESR)
  • Clemence COUTELLE-REBUT (European project manager)
Collaborations
  • Carmen Rivas, USC, Santiago de Compostela, ES,
  • Gael Roué, IDIBAPS-Hospital Clinic of Barcelona, ES,
  • Dimitris Xirodimas, CRBM-CNRS, Montpellier, FR (ITN UbiCODE),
  • Jean-Emmanuel Sarry, CRCT Toulouse, FR,
  • Pierre Lutz, IPBS Toulouse, FR,
  • Rosa Barrio, CIC bioGUNE, Bilbao, ES (ITN UbiCODE),
  • Niels Reichardt, CIC biomaGUNE, San Sebastian, ES,
  • Ron Hay, University of Dundee, Scotland, UK (ITN UbiCODE),
  • Rune Mathiesen, Nova Medical School, Lisboa, PT (ITN UbiCODE),
  • Jean Christophe Rain, Hybrigenics, Paris, FR (ITN UbiCODE).

Our supports

Contact

Send an e-mail

+33 5 82 99 10 26

Cancer Biology, Chemoresistance, Ubiquitin-family members, Ubiquitin-Proteasome System, Autophagy-Lysosome System, Molecular traps, Ubiquitin-traps, SUMO-traps, Microarrays, HTS, Proteomics