The goal of the ANCHOR team is to develop new applications for a live cell DNA biosensor technique based on our recently patented system to investigate several DNA processes. The ANCHOR system is a bipartite system composed of ANCH sequences and OR proteins. ANCH DNA sequences are specifically and independently recognized by OR proteins that nucleate and spread from the ANCH sequence over surrounding DNA. As the OR proteins can be fused to a fluorescent reporter, increased concentration of OR on the ANCH DNA creates a fluorescent focus easily detectable by fluorescence microscopy. We are applying this technology to uncover DNA-based molecular mechanisms in single cells and in high content screening by microscopy on living cells to discover active molecules with broad applications, from bio-production to antiviral activity.
ANCHOR technology is of great interest to the research community working on DNA localization and to pharmaceutical industries at large to test effects of new compounds on cancers and virus infection.